Egg Retrieval, Sperm Collection and Fertilisation in IVF
Egg retrieval is performed before the second phase of an IVF cycle. It occurs when ovarian stimulation is successful. A day and a half after HCG is administered; a gynaecologist will use a needle to puncture the follicles.
Egg retrieval usually takes around half an hour to perform. The process can vary based on the number of follicles present. The whole process will be performed under sedation with a recovery period of around an hour.
Women need to be at the clinic half an hour before the procedure. A cardiograph will then be performed. You can also talk with our staff about any other issues that you may have prior to your procedure.
Women should not eat or drink the night before their procedure
On the day of the egg collection the male partner must give a sperm sample. The semen should be gathered after a three to four day abstinence from sexual activity. Masturbation is the preferred method of collection at the clinic. Your circumstances will be reviewed prior, and a recommendation will be made.
After the collection is completed, the sample is sent to the lab where it is prepared for IVF. The spermatozoa is isolated from other elements in the semen and activated in order to fertilise the mature oocytes.
If masturbation cannot be performed on the day of egg retrieval, a slightly older sample may be used although fresh samples are preferred to ensure the couple have the maximum chances of fertility. In cases where the male infertility is severe, other efforts can be tried after egg retrieval.
In cases of Men that have Azoospermia, spermatozoa are retrieved directly from the testis via surgery.
After egg retrieval and semen prep the IVF will begin. In an IVF cycle the mature oocytes are placed in four dishes containing processed sperm. One of the sperm will fertilise the oocyte to begin the process.
Fertilisation may be assisted by sperm injection (ICSI) which is a technique that involves the injection of sperm directly into the egg. The eggs are then incubated in the lab overnight.
Approximately eighteen hours after the procedure, a check for fertilisation is performed by the embryologists. The first signs of fertilisation are shown through two pronuclei appearing within the egg. The fertilisation rate is usually fifty percent and above. The variation in the success rate can be through things such as the maturity of the oocytes, semen parameters and other factors.